Phosphate → HCID 88811000, compound

HRID 973138Notes

Reaction equation

C(N[C@H](C([NH:14][C@H:15]([C:19]([N:21]([C:39](=[O:48])[CH2:40][CH2:41][CH:42]([NH2:47])[CH2:43][CH:44]([CH3:46])[CH3:45])[C@H:22]([C:25]([CH:27]([CH:31]([OH:38])[CH:32]([NH2:37])[CH2:33][CH:34]([CH3:36])[CH3:35])[C:28]([OH:30])=[O:29])=[O:26])[CH2:23][OH:24])=[O:20])[CH:16]([CH3:18])[CH3:17])=O)C(C)C)(=O)CC(C)C.P([O-])([O-])([O-])=O>>[NH2:14][C@H:15]([C:19]([N:21]([C:39](=[O:48])[CH2:40][CH2:41][CH:42]([NH2:47])[CH2:43][CH:44]([CH3:46])[CH3:45])[C@H:22]([C:25]([CH:27]([CH:31]([OH:38])[CH:32]([NH2:37])[CH2:33][CH:34]([CH3:35])[CH3:36])[C:28]([OH:30])=[O:29])=[O:26])[CH2:23][OH:24])=[O:20])[CH:16]([CH3:18])[CH3:17]

Participants

Reactant 2

HCID 1061Phosphate

HCID 88811214HCID 88811214, compound

Product 1

HCID 88811000HCID 88811000, compoundYield 84.3%

Procedure

A reaction mixture containing 100 mg of isovaleryl-L-valyl-L-valyl-4-amino-3-hydroxy-6-methylheptanoyl-L-alanyl-4-amino-3-hydroxy-6-methylheptanoic acid, 250 units of purified enzyme prepared by the procedure described in Experiment 17 and 0.05M phosphate buffer, pH 7.2, in a total volume of 100 ml was incubated at 37° C for 20 hours. By a procedure similar to that described in Example 1, 61.8 mg of crystalline Val--X--Ala--X was obtained.

Workup

customprepared by the procedure

Sources & evidence

ORD record: ord-6688f788c1e044419ca4393053770d7c
Source dataset: uspto-grants-1977_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04055468

Reaction notes

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