Tacrolimus-13C,D2 → HCID 57266865, compound

Participants

Reaction Steps

To a stirred solution of 17-allyl-1,14-dihydroxy-12-[2'-(4"-hydroxy-3"-methoxycyclohexyl)-1'-methylvinyl]-23,25-dimethoxy-13,19,21,27-tetramethyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9 ]octacos-18-ene-2,3,10,16-tetraone (108 mg, 0.135 mmol) in 4 ml MeOH cooled to -5° C. (ice/acetone bath) was added in several portions NaBH3CN (160 mg). After addition was complete reaction was allowed to warm to RT. After stirring at RT for 2 hours the reaction mixture was diluted with 10 ml CH2Cl2 and purified by prep TLC (5% MeOH/CH2Cl2) to give the title compound (with NMR and mass spectra consistent with the structure).

Provenance & Evidence