Dicyclohexylcarbodiimide → HCID 131715158, compound

HRID 973901Notes

Reaction equation

HRID 973901 reaction equation
C1(N=C=NC2CCCCC2)CCCCC1.[NH2:16][CH2:17][CH2:18][C:19]1[NH:28][C:27]2[C:26](=[O:29])[N:24]([CH3:25])[C:23](=[O:30])[N:22]([CH3:31])[C:21]=2[N:20]=1.[CH:32]1[C:37]([C:38]([OH:40])=[O:39])=[CH:36][C:35]2[C:41]3([O:57][C:58](=[O:59])[C:34]=2[CH:33]=1)[C:51]1[CH:52]=[CH:53][C:54]([OH:56])=[CH:55][C:50]=1[O:49][C:43]1[CH:44]=[C:45]([OH:48])[CH:46]=[CH:47][C:42]3=1>N1C=CC=CC=1>[NH2:16][CH2:17][CH2:18][C:19]1[NH:28][C:27]2[C:26](=[O:29])[N:24]([CH3:25])[C:23](=[O:30])[N:22]([CH3:31])[C:21]=2[N:20]=1.[CH:32]1[C:37]([C:38]([OH:40])=[O:39])=[CH:36][C:35]2[C:41]3([O:57][C:58](=[O:59])[C:34]=2[CH:33]=1)[C:51]1[CH:52]=[CH:53][C:54]([OH:56])=[CH:55][C:50]=1[O:49][C:43]1[CH:44]=[C:45]([OH:48])[CH:46]=[CH:47][C:42]3=1

Participants

5

Reactant 3

HCID 10868DicyclohexylcarbodiimideHCID 768066-CarboxyfluoresceinHCID 4140301AC1N56UX

Product 1

HCID 131715158HCID 131715158, compound

Reagents, catalysts & solvents 1

HCID 1049PyridineSolvent

Procedure

N,N'-dicyclohexylcarbodiimide (15 mg) was added to a solution of 8-β-aminoethyltheophylline (5 mg) and carboxyfluorescein (5 mg) in 0.5 ml of pyridine. The reaction was allowed to proceed for two hours at room temperature and the reaction product was purified twice employing silica gel thin-layer chromatography using a thin chloroform:methanol (2:1) mixture as developing solvent to yield an 8-β-amino ethyltheophylline-carboxyfluorescein conjugate of the formula: ##STR10##

Workup

  1. customthe reaction product was purified twice employing silica gel thin-layer chromatography

Sources & evidence

ORD record
ord-d7912e197081430b82dbad2178b728a4
Source dataset
uspto-grants-1991_11
Patent number
US05066426

Reaction notes

Loading…