Phosphate → 4-Nitrophenol

Beteiligte Stoffe

Reaktionsbedingungen

Reaktionsschritte

With 0.5 ml of a substrate solution containing 20 mM 4-nitrophenyl-α-D-glucopyranoside was mixed 1.0 ml of 0.1M phosphate buffer (pH 7.0), and the mixture was pre-heated at 37° C. for 5 minutes. Thereafter, 0.5 ml of an enzyme solution was added thereto and the reaction was conducted at 37° C. for 5 minutes, followed by determination of changes in absorbance per minute at 400 nm. The enzyme activity producing 1 μmol of 4-nitrophenol per minute under the above-mentioned conditions was taken as one unit.

Herkunft & Nachweise