Sodium Hydroxide → HCID 70008481, compound

HRID 975750Notes

Reaction equation

HRID 975750 reaction equation
[ClH:1].[CH2:2]([NH:6][CH2:7][C@@H:8]([C@H:10]([C@@H:12]([C@@H:14]([CH2:16][OH:17])[OH:15])[OH:13])[OH:11])[OH:9])[CH2:3][CH2:4][CH3:5].[OH-].[Na+]>O>[ClH:1].[CH2:2]([NH:6][CH2:7][C@@H:8]1[O:9][C@:14]([OH:15])([CH2:16][OH:17])[C@@H:12]([OH:13])[C@@H:10]1[OH:11])[CH2:3][CH2:4][CH3:5]

Participants

4

Reactant 2

HCID 14798Sodium HydroxideHCID 22869670HCID 22869670, compound

Product 1

HCID 70008481HCID 70008481, compoundYield 80%

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
15 °C

Procedure

A reaction solution is prepared by adding 109 ml of a bulk solution of N-n-butylglucamine hydrochloride (prepared in Example 1) with a concentration of 500 gm/L by HPLC to 460 ml of water in a 1-L bioreactor. The solution is adjusted to pH 5 by adding dilute sodium hydroxide and cooled to about 15° C. A total of 30 gm (wet weight) of washed G. oxydans cell paste is added and the reaction mixture is agitated (500 rpm) and aerated (0.5 vvm) while controlling the temperature at 15° C. and pH at 5. After 23 hours, the HPLC assay indicates that over 99% of the substrate has been converted for a 80% yield of 6-n-butylamino-6-deoxy-α-L-sorbofuranose hydrochloride.

Workup

  1. customA reaction solution is prepared
  2. additionoxydans cell paste is added
  3. customat 15° C.

Sources & evidence

ORD record
ord-c4206936c13941419117ca7bd2f5d585
Source dataset
uspto-grants-1997_03
Patent number
US05610039

Reaction notes

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