Hydrochloric Acid → HCID 53844535, compound

Participants

Reaction Conditions

Reaction Steps

A reaction solution is prepared by adding 750 gm N-n-butylglucamine to 7.2 L of water in a 10-L bioreactor. The suspension is adjusted to pH 5 (with concentrated HCl ) and cooled to about 15° C. A total of 300 gm (wet weight) of washed G. oxydans cell paste is added and the reaction mixture is agitated (350-500 rpm) and aerated (0.4 vvm) while controlling the temperature at 15° C. and pH at 5. After 14 hours, the HPLC assay indicates that over 97% of the substrate has converted for a 85% yield of 6-n-butylamino-6-deoxy-α-L-sorbofuranose.

Provenance & Evidence