反应详情

N-Benzyloxycarbonyl-D-alanine (2.5 mmol. 0.556 g) and trans-2-methylcyclohexanol (3 equivalents, 1 mL) were dissolved in 7 mL THF at room temperature, followed by the addition of 70 mg 4-dimethylaminopyridine. DCC (2.5 mmol, 0.515 g) was then introduced with stirring. The solution turned cloudy immediately and was stirred at room temperature overnight. The reaction mixture was stirred for another 15 min after several drops of HOAc were added to it. Dicyclohexylurea (DCU) was filtered off and the filtrate evaporated to dryness. The residue was dissolved in ethyl acetate. The EtOAc solution was filtered, washed with 0.5N HCl (2×), brine (2×), 5% NaHCO3 (2×) and finally brine (2×). The organic phase, after being dried over anhydrous Na2SO4, showed one UV-positive tlc spot. A second spot, which could only be detected by phosphomolybdic acid (PMA)--Ce(SO4)2 spray, was identified as the unreacted alcohol by tlc comparison with authentic material. The ethyl acetate solution was evaporated to dryness and dried under high vacuum, giving a clear oil weighing 0.965 g. The crude material was chromatographed on a Merck Si--60 size C prepacked column by gradient elution with toluene to toluene:ethyl acetate (9:1). After evaporation, 0.629 g (72.0%) of clear, colorless oil was recovered: nmr (CDCl3, ppm), 0.85-2.05 (13H, m, aliphatic H of cyclohexanol), 0.9 (3H, d, J=6 Hz, CH3) 1.3 (3H, d, J=7 Hz, obscured by other aliphatic protons, β--CH3 of Ala), 4.3 (2H, m, CH--O-- and α--H), 5 (2H, s, ArCH2 --), 5.25 (1H, broad s, amide H), 7.15 (5H, s, ArH5).

1. stirringwas stirred at room temperature overnight
2. additionwere added to it
3. filtrationDicyclohexylurea (DCU) was filtered off
4. customthe filtrate evaporated to dryness
5. dissolutionThe residue was dissolved in ethyl acetate
6. filtrationThe EtOAc solution was filtered
7. washwashed with 0.5N HCl (2×), brine (2×), 5% NaHCO3 (2×) and finally brine (2×)
8. dry with materialThe organic phase, after being dried over anhydrous Na2SO4
9. customThe ethyl acetate solution was evaporated to dryness
10. customdried under high vacuum
11. customgiving a clear oil
12. customThe crude material was chromatographed on a Merck Si
13. wash60 size C prepacked column by gradient elution with toluene to toluene:ethyl acetate (9:1)
14. customAfter evaporation, 0.629 g (72.0%) of clear, colorless oil
15. customwas recovered