三（羟甲基）氨基甲烷 → D-(-)-Fructose

HRID 223585

反应方程式

HRID 223585 反应方程式
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].C(O)C(N)(CO)CO.C(O)(=O)/C=C\C(O)=O.OS([O-])(=O)=O.[Na+]>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

参与物

5

反应物 4

HCID 6503三（羟甲基）氨基甲烷HCID 107526D-葡萄糖-UL-14C2 次HCID 444266(Z)-丁烯二酸HCID 516919硫酸氢钠

产物 1

HCID 5984D-(-)-Fructose

实验过程

The glucose isomerase activity of the immobilized samples was estimated at 70° C. using a batch assay technique using small particles (≈80 mesh). The assay was carried out in 125 ml. Erlenmeyer flasks in which the dry enzyme was hydrated in substrate (1 M glucose, 0.1 M Tris, 0.1 M maleic acid, 4.1 mM MgSO4.7H2O and 5 mmM NaHSO4 at pH 8.0) for 10 minutes at room temperature. The assay reaction was started by placing the flask in a 70° C. shaker water bath. At 15, 30, 45, 60 and 75 minutes, a 0.05 ml. sample of the reaction mixture was removed and added to 1.0 ml. of 0.1M HClO4 solution to stop the reaction. The amount of fructose formed in each sample was then estimated by the cysteine H2SO4 method. The activity was calculated through regression analysis of fructose formed with respect to time, where a unit of activity represents one μ mole of fructose formed per minute under conditions of the assay.

后处理

  1. customwas estimated at 70° C.
  2. customThe assay reaction
  3. customin a 70° C.
  4. customAt 15, 30, 45, 60 and 75 minutes
  5. customsample of the reaction mixture was removed
  6. additionadded to 1.0 ml
  7. customthe reaction

来源与证据

ORD记录
ord-694cc5f170234a7ebc4259aab84863ab
来源数据集
uspto-grants-1982_10
专利号
US04355105

关联笔记

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