Deferoxamine → HCID 86583864, Verbindung

HRID 1061480

Reaktionsgleichung

Reaktionsgleichung von HRID 1061480
[CH3:1][C:2]([N:4]([OH:39])[CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][NH:10][C:11]([CH2:13][CH2:14][C:15]([N:17]([OH:38])[CH2:18][CH2:19][CH2:20][CH2:21][CH2:22][NH:23][C:24]([CH2:26][CH2:27][C:28]([N:30]([OH:37])[CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][NH2:36])=[O:29])=[O:25])=[O:16])=[O:12])=[O:3].[CH:40]1[C:45](N=C=S)=[CH:44][C:43]2[C:49]([O:51][C:52]3([C:62]4[CH:63]=[CH:64][C:65]([OH:67])=[CH:66][C:61]=4[O:60][C:54]4[CH:55]=[C:56]([OH:59])[CH:57]=[CH:58][C:53]3=4)[C:42]=2[CH:41]=1)=[O:50]>[Al]>[CH:40]1[CH:45]=[CH:44][C:43]([C:49]([OH:51])=[O:50])=[C:42]([C:52]2[C:53]3[CH:58]=[CH:57][C:56]([OH:59])=[CH:55][C:54]=3[O:60][C:61]3[C:62]=2[CH:63]=[CH:64][C:65]([CH:66]=3)=[O:67])[CH:41]=1.[CH3:1][C:2]([N:4]([OH:39])[CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][NH:10][C:11]([CH2:13][CH2:14][C:15]([N:17]([OH:38])[CH2:18][CH2:19][CH2:20][CH2:21][CH2:22][NH:23][C:24]([CH2:26][CH2:27][C:28]([N:30]([OH:37])[CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][NH2:36])=[O:29])=[O:25])=[O:16])=[O:12])=[O:3]

Komponenten

4

Edukt 2

HCID 2973Deferoxamine2×HCID 18730Fluorescein 5-isothiocyanate2×

Produkt 1

HCID 86583864HCID 86583864, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 5359268AluminumLösungsmittel

Versuchsvorschrift

An aliquot of 120 mg of Desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 ml of 0.1 M Na carvonate pH 9.5. A molar ratio of 3:1 of FITC: Desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine, the mixture is electrophoresed over a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 ml fractions. The fluorescein-Desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes.

Aufarbeitung

  1. customto react for at least 24 hours at room temperature
  2. customTo separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine
  3. washare eluted in 2 ml fractions
  4. washThe fluorescein-Desferrioxamine is eluted in the void volume of the column
  5. customThe first peak is collected
  6. customused for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes

Quellen und Nachweise

ORD-Eintrag
ord-d6f5b387ebd944f0835dcb70f656f92a
Quelldatensatz
uspto-grants-2005_11
Patentnummer
US06960560B2

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