Hydrochloric Acid → [(4S,6R,9E,11R,12R)-11-[(2R,3R,4R,5R,6R)-4,5-dihydroxy-6-methyl-3-(methylamino)oxan-2-yl]oxy-4-(2-oxo-1,3-dioxolan-4-yl)-5-oxatricyclo[8.3.0.04,6]trideca-1(13),9-dien-2,7-diyn-12-yl] 2-hydroxy-7-methoxy-5-methylnaphthalene-1-carboxylate

Beteiligte Stoffe

Reaktionsschritte

Into 1.5 l of a 95% methanol solution was suspended 16 g of neocarzinostatin powder. After adjusting the pH of the suspension with 1 N hydrochloric acid to pH 2.0, the suspension was stirred for 2 hours and then subjected to centrifugal separation. The supernatant was then concentrated and dried to 920 mg of crude powder of the NCS-C substance. The crude powder was subjected to Sephadex LH-20 chromatography in the same manner as in Example 3. The NCS-C substance was obtained in a powder form (yield: 105 mg).

Herkunft & Nachweise