Thymine → 5-Methyluridine

HRID 1078177

Reaktionsgleichung

Reaktionsgleichung von HRID 1078177
[CH3:1][C:2]1[C:3](=[O:9])[NH:4][C:5](=[O:8])[NH:6][CH:7]=1.[C@@H:10]1(N2C3N=C(N)NC(=O)C=3N=C2)[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1[OH:12]>>[CH3:1][C:2]1[C:3](=[O:9])[NH:4][C:5](=[O:8])[N:6]([CH:7]=1)[C@@H:10]1[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1[OH:12]

Komponenten

3

Edukt 2

HCID 1135ThymineHCID 135398635Guanosine

Produkt 1

HCID 4454085-Methyluridine

Versuchsvorschrift

A culture broth of Flavobacterium rhenanum FERM BP-1862 was prepared according to Example 1 of JP 2-23882. 5-Methyluracil and guanosine as substrates were added without removing the culture medium ingredients of the strain culture solution, and were reacted while keeping the temperature at 60° C. in the same manner as in Example 1 of the present invention. The reaction solution was cooled, filtered, decolored with activated carbon, refiltered and concentrated in vacuo. The concentrate was recooled to 5° C. The particle diameter of the obtained 5-methyluridine crystals was from 20 to 30 μm. The separation phenomenon of 5-methyluridine crystals from other crystals of nucleic acids formed as impurities was not observed. Further, the centrifugal separation took much time.

Aufarbeitung

  1. customA culture broth of Flavobacterium rhenanum FERM BP-1862 was prepared
  2. customwithout removing the culture medium ingredients of the strain culture solution
  3. customwere reacted
  4. customat 60° C.
  5. temperatureThe reaction solution was cooled
  6. filtrationfiltered
  7. concentrationconcentrated in vacuo
  8. customwas recooled to 5° C

Quellen und Nachweise

ORD-Eintrag
ord-2fa94e55415d4fbc9f02525159d50768
Quelldatensatz
uspto-grants-1996_08
Patentnummer
US05547857

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