Prostaglandin D2 → Arachidonic Acid

Beteiligte Stoffe

Reaktionsschritte

The thus obtained acidic liquid was extracted 3 times with each 5 ml of ether, and after evaporating ether off from the ether extract, the dried solid was subjected to esterification by a diazomethane solution. The product of esterification was fractioned by thin layer chromatography while developing with a 90:50:20:100 by volume mixture of ethyl acetate, isooctane, acetic acid and water to obtained fractioned spots which were identified by the authentic specimens of PGD2, PGE2, PGF2α and 6-keto-PGF1α.

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