HRID1085044

反应详情

EQUATION

反应方程式

REACTANTS

反应物

$结构：CCCCC[C@H](O)/C=C/[C@H]1C(=O)C[C@H](O)[C@@H]1C/C=C\CCCC(=O)O$

HCID448457

Prostaglandin D2

C20H32O5

$结构：CCCCC[C@H](O)/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O$

HCID5280360

未命名化合物

$结构：CCCCC[C@H](O)/C=C/[C@@H]1[C@@H](C/C=C\CCCC(=O)O)[C@@H](O)C[C@H]1O$

HCID5280363

Dinoprost

C20H34O5

结构：CCCCC[C@H](O)/C=C/[C@@H]1[C@@H](CC(=O)CCCCC(=O)O)[C@@H](O)C[C@H]1O

HCID5280888

6-Keto-prostaglandin F1alpha

C20H34O6

PRODUCTS

生成物

$结构：CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)O$

HCID444899

Arachidonic acid

C20H32O2

PROCEDURE

实验过程

The thus obtained acidic liquid was extracted 3 times with each 5 ml of ether, and after evaporating ether off from the ether extract, the dried solid was subjected to esterification by a diazomethane solution. The product of esterification was fractioned by thin layer chromatography while developing with a 90:50:20:100 by volume mixture of ethyl acetate, isooctane, acetic acid and water to obtained fractioned spots which were identified by the authentic specimens of PGD2, PGE2, PGF2α and 6-keto-PGF1α.

WORKUP

后处理

extractionThe thus obtained acidic liquid was extracted 3 times with each 5 ml of ether
customafter evaporating ether off from the ether
extractionextract
additionwhile developing with a 90:50:20:100 by volume mixture of ethyl acetate, isooctane, acetic acid and water
customto obtained fractioned spots which