Water → 4-((2S)-2-((5-Isoquinolinylsulfonyl)methylamino)-3-oxo-3-(4-phenyl-1-piperazinyl)propyl)phenyl 5-isoquinolinesulfonate

2.27 g of the amorphous compound obtained in Example 108 was dissolved in 30 ml of dimethylformamide, to the solution were sequentially added 160 mg of 60% sodium hydride and 0.3 ml of methyl iodide with ice cooling, and the mixture was stirred for 90 minutes with ice cooling. After adding 80 ml of water, the reaction mixture was extracted with 100 ml of ethyl acetate, and the extract was washed with 80 ml of saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (60:1), to obtain 1.8 g of the title compound in a yellow amorphous form.

1. extractionthe reaction mixture was extracted with 100 ml of ethyl acetate
2. washthe extract was washed with 80 ml of saturated sodium chloride aqueous solution
3. dry with materialdried over magnesium sulfate
4. concentrationconcentrated under a reduced pressure
5. washeluted with chloroform/methanol (60:1)

ORD-Eintrag

ord-4b7cb21fc1664fc4891e130169053ef7

Quelldatensatz

uspto-grants-1992_01

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US05081246