Methyl Ethyl Ketone → 17alpha-Hydroxy-21-butanoyl-pregna-4-ene-3,20-dione

HRID 1097187Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1097187
[OH:1][C@:2]1([C@:23]2([CH3:24])[C@H:9]([C@H:10]3[C@H:20]([CH2:21][CH2:22]2)[C@:18]2([CH3:19])[C:13](=[CH:14][C:15](=[O:25])[CH2:16][CH2:17]2)[CH2:12][CH2:11]3)[CH2:8][CH2:7]1)[C:3](=[O:6])CO.[C:26]([O:31]CC(F)(F)F)(=O)[CH2:27][CH2:28][CH3:29].[CH2:37](C(C)=O)C>>[OH:1][C@:2]1([C@:23]2([CH3:24])[C@H:9]([C@H:10]3[C@H:20]([CH2:21][CH2:22]2)[C@:18]2([CH3:19])[C:13](=[CH:14][C:15](=[O:25])[CH2:16][CH2:17]2)[CH2:12][CH2:11]3)[CH2:8][CH2:7]1)[C:3](=[O:6])[CH2:37][C:26](=[O:31])[CH2:27][CH2:28][CH3:29]

Komponenten

4

Edukt 3

HCID 6569Methyl Ethyl KetoneHCID 1932902,2,2-Trifluoroethyl butanoateHCID 44070711-Deoxycortisol

Produkt 1

HCID 6681263217alpha-Hydroxy-21-butanoyl-pregna-4-ene-3,20-dione

Versuchsvorschrift

A mixture of 1 g of 17α,21-dihydroxy-pregna-4-ene-3,20-dione and 10 ml of trifluoroethyl butanoate in 50 ml of methyl ethyl ketone was reacted at 45° C. in the presence of 5 g of Candida cylindracea lipase for 8-10 hours, adding at regular time intervals 1 g of lipase. After completion of the reaction, the lipase was filtered off, the filtrate was concentrated under vacuum, taking up the residue three times with solvent. The semi-solid residue was purified by chromatography on a silica gel column with a dichloromethane/methanol 99:1 mixture. The richer fraction was evaporated to obtain 0.89 g (2.14 mM) of 17α-hydroxy-21-butanoyl-pregna-4-ene-3,20-dione.

Aufarbeitung

  1. customwas reacted at 45° C. in the presence of 5 g of Candida cylindracea lipase for 8-10 hours
  2. additionadding at regular time intervals 1 g of lipase
  3. customAfter completion of the reaction
  4. filtrationthe lipase was filtered off
  5. concentrationthe filtrate was concentrated under vacuum
  6. customThe semi-solid residue was purified by chromatography on a silica gel column with a dichloromethane/methanol 99:1 mixture
  7. customThe richer fraction was evaporated

Quellen und Nachweise

ORD-Eintrag
ord-6b5753c446f1479aa599ba3763c8613e
Quelldatensatz
uspto-grants-2012_03
Patentnummer
US08143240B2

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