Phosphate → aldehydo-D-Xylose

Temperatur

60 °C

Xylose (100 gm) was dissolved in distilled water to provide a concentration of 60% w/v. The solution was placed in a reaction vessel to which was added CoCl2 and MgCl2 to provide a concentration of Co++ =10-4M and Mg++ =10-3M. The pH of the solution was adjusted to 7.5 by the addition of a phosphate buffer. To the resulting solution was then added 5 gm of glucose isomerase extracted from Streptomyces and the temperature was maintained at 60° C. overnight (18 hours). The solution was then added to a fermentation medium (DIFCO 8.5 gm/l yeast extract, NH4Cl1.3 gm/l, MgSO4 ·7 H2O·11 gm/l, and CaCl2 .06 gm/l) to yield an initial fermentation substrate concentration of 78 gm/l xylose and 20 gm/l xylulose. The resulting pH was 5.5.

1. customto provide a concentration of 60% w/v
2. customThe solution was placed in a reaction vessel to which
3. customto provide a concentration of Co++ =10-4M and Mg++ =10-3M
4. extractionextracted from Streptomyces
5. additionThe solution was then added to a fermentation medium (DIFCO 8.5 gm/l yeast extract, NH4Cl1.3 gm/l, MgSO4 ·7 H2O·11 gm/l, and CaCl2 .06 gm/l)

ORD-Eintrag

ord-e6eea9964d9a4e8896b2697077a4e883

Quelldatensatz

uspto-grants-1984_12

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US04490468