Acetate → ASIALOGANGLIOSIDE-GM1

HRID 1197403Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1197403
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][C@H:21]([C@H:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][C@@H:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([O:67][C@@]3(C(O)=O)O[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(C)=O)[C@@H](O)C3)[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19].C([O-])(=O)C>O>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][CH:21]([CH:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][CH:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([OH:67])[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19]

Komponenten

4

Edukt 2

HCID 175AcetateHCID 9963963Ganglioside GM1

Produkt 1

HCID 6450363ASIALOGANGLIOSIDE-GM1

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Versuchsvorschrift

A 10 ml quantity of 0.05% ganglioside GM1 solution, 10 ml of 40 mM acetate buffer (pH 5.0) and 10 ml of sterilized water were mixed together, and to the mixture was added 10 ml of aqueous Isozyme L (3 U/ml), followed by reaction at 37° C. for 8 hours. The reaction mixture was subjected to purification in the same manner as in Example 1, giving about 2.6 mg of asialo GM1 as a white powder. The production of asialo GM1 was confirmed in the same manner as in Example 1.

Aufarbeitung

  1. additionto the mixture was added 10 ml of aqueous Isozyme L (3 U/ml)
  2. customfollowed by reaction at 37° C. for 8 hours
  3. customThe reaction mixture was subjected to purification in the same manner as in Example 1

Quellen und Nachweise

ORD-Eintrag
ord-8521938072454751abdfcfe23566ef80
Quelldatensatz
uspto-grants-1994_01
Patentnummer
US05275939

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