Terbinafine Hydrochloride → Terbinafine

HRID 1244179

Reaktionsgleichung

Reaktionsgleichung von HRID 1244179
[CH3:1][C:2]([C:5]#[C:6]/[CH:7]=[CH:8]/[CH2:9][N:10]([CH2:12][C:13]1[CH:14]=[CH:15][CH:16]=[C:17]2[CH:22]=[CH:21][CH:20]=[CH:19][C:18]=12)[CH3:11])([CH3:4])[CH3:3].Cl>C(=O)([O-])[O-]>[CH3:4][C:2]([C:5]#[C:6]/[CH:7]=[CH:8]/[CH2:9][N:10]([CH2:12][C:13]1[CH:14]=[CH:15][CH:16]=[C:17]2[CH:22]=[CH:21][CH:20]=[CH:19][C:18]=12)[CH3:11])([CH3:1])[CH3:3]

Komponenten

3

Edukt 1

HCID 5282481Terbinafine Hydrochloride

Produkt 1

HCID 1549008Terbinafine

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 19660Carbonate IonLösungsmittel

Versuchsvorschrift

Microtiter plates are coated with 5 μg/ml terbinafine hydrochloride—protein (BSA) conjugate in carbonate buffer overnight at 4°, then saturated with blocking reagent [SuperBloc® buffer (Pierce)] and washed 3× with 0.05% (v/v) PBS-TWEEN® (polyoxyethylene 20 sorbitan monoleate). The hybridoma supernatants to be screened are diluted in a 1% (w/v) solution of BSA in PBS-TWEEN® (polyoxyethylene 20 sorbitan monoleate), and incubated for 2 hours at 37°. Level of bound antibody is measured by anti-mouse IgG rabbit immunoglobulin coupled to horseradish peroxidase with OPD as the substrate. After incubation for one hour at room temperature the enzymatic substrate is hydrolyzed and absorbance at 490/650 nm is measured after 15 minutes incubation in the dark.

Aufarbeitung

  1. washsaturated with blocking reagent [SuperBloc® buffer (Pierce)] and washed 3× with 0.05% (v/v) PBS-TWEEN® (polyoxyethylene 20 sorbitan monoleate)
  2. additionThe hybridoma supernatants to be screened are diluted in a 1% (w/v) solution of BSA in PBS-TWEEN® (polyoxyethylene 20 sorbitan monoleate)
  3. waitAfter incubation for one hour at room temperature the enzymatic substrate is hydrolyzed
  4. waitabsorbance at 490/650 nm is measured after 15 minutes incubation in the dark

Quellen und Nachweise

ORD-Eintrag
ord-264059eabc304be78d9bf253c3e9b904
Quelldatensatz
uspto-grants-2006_10
Patentnummer
US07122184B2

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