Diisopropylethylamine → HCID 131726332, Verbindung

HRID 1271012

Reaktionsgleichung

Reaktionsgleichung von HRID 1271012
FC1C([O:8][C:9](=O)[C@@H:10]2[CH2:14][CH2:13][CH2:12][N:11]2C(OCC2C3C(=CC=CC=3)C3C2=CC=CC=3)=O)=C(F)C(F)=C(F)C=1F.CCN(C(C)C)C(C)C.[NH2:46][CH2:47][C:48]([NH:50][C@H:51]([C:59]([NH:61][C@H:62]([C:67]([OH:69])=[O:68])[CH2:63][CH:64]([CH3:66])[CH3:65])=[O:60])[CH2:52][C:53]1[CH:58]=[CH:57][CH:56]=[CH:55][CH:54]=1)=[O:49]>CN(C=O)C>[NH:11]1[CH2:12][CH2:13][CH2:14][C@H:10]1[C:9]([NH:46][CH2:47][C:48]([NH:50][C@H:51]([C:59]([NH:61][C@H:62]([C:67]([OH:69])=[O:68])[CH2:63][CH:64]([CH3:65])[CH3:66])=[O:60])[CH2:52][C:53]1[CH:58]=[CH:57][CH:56]=[CH:55][CH:54]=1)=[O:49])=[O:8]

Komponenten

5

Edukt 3

HCID 81531DiisopropylethylamineHCID 2733412Fmoc-Pro-OPfpHCID 7016074H-Gly-phe-leu-OH

Produkt 1

HCID 131726332HCID 131726332, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 6228DimethylformamideLösungsmittel

Versuchsvorschrift

Fmoc-L-proline pentafluorophenyl ester (50 mg, 0.1 mmol) is dissolved in anhydrous DMF (1 mL) containing DIEA (17 μL, 0.1 mmol). About 0.5-1.0 mL of this solution is added to Gly-L-Phe-L-Leu ELAMS™ in a test tube and the tube vortexed for about 3 hours. The ELAMS™ are pelleted and washed with DMF (4×) and methylene chloride (2×). Deprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min. The ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×) and dried at 60° C. for 4 hours. The identifier tag for each ELAMS™ is detected and recorded.

Aufarbeitung

  1. washwashed with DMF (4×) and methylene chloride (2×)
  2. waitDeprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min
  3. washThe ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×)
  4. customdried at 60° C. for 4 hours

Quellen und Nachweise

ORD-Eintrag
ord-d21f095c9ab2429eb6fd893ae2687b51
Quelldatensatz
uspto-grants-2007_04
Patentnummer
USRE039571E1

Verknüpfte Notizen

Wird geladen…