Sodium Chloride → HCID 178419078, Verbindung

HRID 1292724

Reaktionsgleichung

Reaktionsgleichung von HRID 1292724
[Na+].[Cl-].C[N+](CCSS(C)(=O)=[O:11])(C)C.[Br-].CCCCC[CH2:20][N:21]=[C:22]([N:24]=[C:25](N)N)N.C(S)[C@@H](O)[C@H](O)CS.C([N:39]([C:43]([CH2:48]O)([CH2:46][OH:47])CO)CCO)CO>>[NH2:39][C@H:43]([C:46]([OH:47])=[O:11])[CH2:48][C:20]1[N:21]=[CH:22][NH:24][CH:25]=1

Komponenten

6

Edukt 5

HCID 5234Sodium ChlorideHCID 20977N-Hexylimidodicarbonimidic diamideHCID 81462BistrisHCID 107933(2-(Trimethylammonium)ethyl)methanethiosulfonateHCID 446094D-1,4-Dithiothreitol

Produkt 1

HCID 178419078HCID 178419078, Verbindung

Versuchsvorschrift

Reactions were assembled in a volume of 260 μl containing 50 mM Hoes buffer, 150 mM NaCl, pH 7.5 and as indicated 5 μM SrtA-DN in 50 mM BisTris, pH 7.5, 10 AM LPXTG (SEQ ID NO: 1) peptide (DABCYL-QALPETGEE-EDANS) (QALPETGEE is SEQ. ID NO: 39), 10 μM TGXLP (SEQ ID NO 45) peptide (DABCYL-QATGELPEE-EDANS) (QATGELPEE is SEQ ID NO: 46), 5 mM MTSET, 0.2 M NH-2-OH, 5 mM pHMB or 10 mM DTT. Incubations were carried out for 1 hour at 37° C. Samples were analyzed in a fluorimeter using 395 nm for excitation and 495 nm for recordings.

Aufarbeitung

  1. additioncontaining 50 mM Hoes buffer

Quellen und Nachweise

ORD-Eintrag
ord-9e2f6feba890452186d53b161d56bba5
Quelldatensatz
uspto-grants-2007_07
Patentnummer
US07238489B2

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