Diisopropylethylamine → 60254-83-3

HRID 1348498

Reaktionsgleichung

Reaktionsgleichung von HRID 1348498
FC1C([O:8][C:9](=O)[CH2:10][NH:11]C(OCC2C3C(=CC=CC=3)C3C2=CC=CC=3)=O)=C(F)C(F)=C(F)C=1F.CCN(C(C)C)C(C)C.[NH2:43][CH2:44][C:45]([NH:47][C@H:48]([C:56]([NH:58][C@H:59]([C:64]([OH:66])=[O:65])[CH2:60][CH:61]([CH3:63])[CH3:62])=[O:57])[CH2:49][C:50]1[CH:55]=[CH:54][CH:53]=[CH:52][CH:51]=1)=[O:46]>CN(C=O)C>[NH2:11][CH2:10][C:9]([NH:43][CH2:44][C:45]([NH:47][C@H:48]([C:56]([NH:58][C@H:59]([C:64]([OH:66])=[O:65])[CH2:60][CH:61]([CH3:62])[CH3:63])=[O:57])[CH2:49][C:50]1[CH:55]=[CH:54][CH:53]=[CH:52][CH:51]=1)=[O:46])=[O:8]

Komponenten

5

Edukt 3

HCID 81531DiisopropylethylamineHCID 2733282Fmoc-Gly-OPfpHCID 7016074HCID 7016074, Verbindung

Produkt 1

HCID 15161060254-83-3

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 6228DimethylformamideLösungsmittel

Versuchsvorschrift

Fmoc-glycine pentafluorophenyl ester (46 mg, 0.1 mmol) is dissolved in anhydrous DMF (1 mL) containing DIEA (17 μL, 0.1 mmol). About 0.5-1.0 mL of this solution is added to Gly-L-Phe-L-Leu ELAMS™ in a test tube and the tube vortexed for about 3 hours. The ELAMS™ are pelleted and washed with DMF (4×) and methylene chloride (2×). Deprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min. The ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×) and dried at 60° C. for 4 hours. The identifier tag for each ELAMS™ is detected and recorded.

Aufarbeitung

  1. washwashed with DMF (4×) and methylene chloride (2×)
  2. waitDeprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min
  3. washThe ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×)
  4. customdried at 60° C. for 4 hours

Quellen und Nachweise

ORD-Eintrag
ord-3db42bfb6eac4dc7860f0e2979cc25f3
Quelldatensatz
uspto-grants-2007_05
Patentnummer
USRE039606E1

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