Carbonate Ion → Biotin

HRID 14354

Reaktionsgleichung

Reaktionsgleichung von HRID 14354
C1C(=O)N(OC(CCCCCN[C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@@H:23]2[S:27][CH2:26][C@@H:25]3[NH:28][C:29]([NH:31][C@H:24]23)=[O:30])=[O:18])=O)C(=O)C1.C(=O)([O-])[O-:33]>>[OH:33][C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@H:23]1[C@@H:24]2[C@@H:25]([NH:28][C:29]([NH:31]2)=[O:30])[CH2:26][S:27]1)=[O:18]

Komponenten

3

Edukt 2

HCID 19660Carbonate IonHCID 83874Nhs-LC-biotin2×

Produkt 1

HCID 171548Biotin

Versuchsvorschrift

Following the above-mentioned method, labeled antibodies were produced using monoclonal antibodies. Anti-α-subunit monoclonal antibody (IM0365) (5 μg/mL) dissolved in 0.1 M carbonate buffer (pH 8.5) and NHS-LC-BIOTIN (PIERCE, 25 μg/mL) were mixed and stirred at room temperature for four hours using a stirrer. IgG and NHS-LC-BIOTIN were mixed at a mole ratio of 1:60. After stiring, this solution was dialyzed against physiological phosphate buffered saline (PBS) to obtain biotin-labeled antibodies.

Aufarbeitung

  1. customwere produced
  2. customthis solution was dialyzed against physiological phosphate buffered saline (PBS)

Quellen und Nachweise

ORD-Eintrag
ord-fc1ecf2b5bb74b8f99d6330e850500cb
Quelldatensatz
uspto-grants-2010_06
Patentnummer
US07732154B2

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