Sucrose → (2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

HRID 14365

Reaktionsgleichung

Reaktionsgleichung von HRID 14365
C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.[CH3:24][NH:25][C@@H:26]1[C@@H:31]([OH:32])[C@H:30]([O:33][C@@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C:44]4([O:50][C@H:49]([C@@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@H:35]23)[C@@H:29]([OH:58])[C@H:28]([NH2:59])[CH2:27]1>>[CH3:24][NH:25][C@H:26]1[C@H:31]([OH:32])[C@@H:30]([O:33][C@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C@:44]4([O:50][C@H:49]([C@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@@H:35]23)[C@H:29]([OH:58])[C@@H:28]([NH2:59])[CH2:27]1

Komponenten

3

Edukt 2

HCID 5988SucroseHCID 35766Hygrovetine

Produkt 1

HCID 52949613(2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

Versuchsvorschrift

After completion of the electroshock, 1 ml of Vogel's minimal medium that contained 1.2% sucrose was added to the resultant, and the obtained mixture was then incubated at 30° C. for 2 hours. 200 μl of the obtained solution was applied to and expanded on an agar medium that contained hygromycin B (500 μg/ml). A hygromycin-resistance colony was isolated, and it was then confirmed by PCR whether or not substitution took place in the target locus. In addition, it was also confirmed by the Southern blot method whether or not it contained redundant Hygr gene copies.

Aufarbeitung

  1. additionwas added to the resultant

Quellen und Nachweise

ORD-Eintrag
ord-0abf829f876f400f8a0f7cb628a03d6a
Quelldatensatz
uspto-grants-2010_06
Patentnummer
US07732208B2

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