Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|
| Edukt | Uridine Diphosphate | HCID 6031 | — |
| Edukt | Bucladesine | HCID 9687 | — |
| Edukt | Udp-Galactose | HCID 18068 | — |
| Edukt | X 100 | HCID 19153 | — |
| Edukt | Phorbol 12-myristate 13-acetate | HCID 27924 | — |
| Edukt | 8-bromo-cAMP | HCID 32014 | — |
| Edukt | N-acetyl-alpha-D-galactosamine | HCID 84265 | — |
| Edukt | Uridine-Diphosphate-N-Acetylglucosamine | HCID 445675 | — |
| Edukt | Monobasic potassium phosphate | HCID 516951 | — |
| Edukt | (2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 3037556 | — |
| Edukt | beta-D-Gal-(1->3)-alpha-D-GalNAc | HCID 5288347 | — |
| Edukt | HCID 178513008 | HCID 178513008 | — |
| Lösungsmittel | Acetonitrile | HCID 6342 | — |
| Produkt | N-Acetyl-D-Glucosamine | HCID 439174 | — |
Reaktionsschritte
Galβ1-3GalNAcα-pNp and GalNAcα-pNp, H-7, H-8 and H-1004 were purchased from Toronto Research Chemicals, Toronto, Canada, UDP-Gal, UDP-GlcNAc, bovine milk β1-4Gal-T (defined as 5-15 units/mg in the presence of lactalbumin), buffer salts and Triton X-100, cholera toxin, dibutyryl cAMP, 8-bromo-cAMP and phorbol 12-myristate 13-acetate (PMA) and TDA were obtained from Sigma. HPLC grade KH2PO4 and acetonitrile were obtained from Fisher Scientific. UDP-6- 3H!-galactose (18.9 Ci/mmol) and UDP-6- 3H!-N-acetylglucosamine (26.8 Ci/mmol) were provided by Amersham and NEN, respectively. C18 Sep-Pak were from Millipore-Waters. Actinomycin D and cycloheximide were purchased from Calbiochem. UDP-6- 3H!-galactose (18.9 Ci/mmole) was purchased from Amersham; dimethysulfoxide (DMSO) from BDH; all-trans-retinoic acid and sodium butyrate from Aldrich; α-modified Eagle's minimum essential medium (α-MEM) and fetal calf serum from Gibco.
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patentnummer | US05501957 |
|---|
| Quelldatensatz | uspto-grants-1996_03 |
|---|
| ORD ID | ord-680e10130a674bda9d787e67e62d5f31 |
|---|
| Quellennotiz | uspto-grants-1996_03 |
|---|
