HRID1440957

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=c1ccn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID6031

未命名化合物

2 次

结构：CCCC(=O)Nc1ncnc2c1ncn2[C@@H]1O[C@@H]2COP(=O)(O)O[C@H]2[C@H]1OC(=O)CCC

HCID9687

Bucladesine

C18H24N5O8P

结构：O=c1ccn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID18068

Uridine diphosphate galactose

C15H24N2O17P2

结构：CCC(CC)COC(C(=O)N(C)CC[NH+](C)C)(c1ccccc1)c1ccccc1.[Cl-]

HCID19153

未命名化合物

结构：CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)[C@@H](C=C(CO)C[C@]3(O)C(=O)C(C)=C[C@@H]23)[C@@H]2C(C)(C)[C@]12OC(C)=O

HCID27924

Phorbol 12-myristate 13-acetate

C36H56O8

结构：Nc1ncnc2c1nc(Br)n2[C@@H]1O[C@@H]2COP(=O)(O)O[C@H]2[C@H]1O

HCID32014

未命名化合物

结构：CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O

HCID84265

.alpha.-D-Galactopyranose, 2-(acetylamino)-2-deoxy-

C8H15NO6

结构：CC(=O)N[C@H]1[C@@H](OP(=O)(O)OP(=O)(O)OC[C@H]2O[C@@H](n3ccc(=O)[nH]c3=O)[C@H](O)[C@@H]2O)O[C@H](CO)[C@@H](O)[C@@H]1O

HCID445675

Uridine-diphosphate-N-acetylglucosamine

C17H27N3O17P2

HCID516951

Monobasic potassium phosphate

H2KO4P

结构：O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO

HCID3037556

(2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal

C6H12O6

结构：CC(=O)N[C@@H]1[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H](CO)O[C@@H]1O

HCID5288347

未命名化合物

HCID178513008

未命名化合物

PRODUCTS

生成物

结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O

HCID439174

N-Acetylglucosamine cyclized form

C8H15NO6

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID6342

Acetonitrile

C2H3N

PROCEDURE

实验过程

Galβ1-3GalNAcα-pNp and GalNAcα-pNp, H-7, H-8 and H-1004 were purchased from Toronto Research Chemicals, Toronto, Canada, UDP-Gal, UDP-GlcNAc, bovine milk β1-4Gal-T (defined as 5-15 units/mg in the presence of lactalbumin), buffer salts and Triton X-100, cholera toxin, dibutyryl cAMP, 8-bromo-cAMP and phorbol 12-myristate 13-acetate (PMA) and TDA were obtained from Sigma. HPLC grade KH2PO4 and acetonitrile were obtained from Fisher Scientific. UDP-6- 3H!-galactose (18.9 Ci/mmol) and UDP-6- 3H!-N-acetylglucosamine (26.8 Ci/mmol) were provided by Amersham and NEN, respectively. C18 Sep-Pak were from Millipore-Waters. Actinomycin D and cycloheximide were purchased from Calbiochem. UDP-6- 3H!-galactose (18.9 Ci/mmole) was purchased from Amersham; dimethysulfoxide (DMSO) from BDH; all-trans-retinoic acid and sodium butyrate from Aldrich; α-modified Eagle's minimum essential medium (α-MEM) and fetal calf serum from Gibco.

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-680e10130a674bda9d787e67e62d5f31
来源数据集: uspto-grants-1996_03
DOI: 10.6084/m9.figshare.5104873.v1
专利: US05501957
来源说明: uspto-grants-1996_03

反应 SMILES

[O:1]([C@H:13]1[C@@H:19]([OH:20])[C@@H:18]([CH2:21][OH:22])[O:17][C@H:15]([OH:16])[C@@H:14]1[NH:23][C:24]([CH3:26])=[O:25])[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O.O[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1NC(C)=O.C1C=C2C(=C(S(NCCNC(N)=N)(=O)=O)C=1)C=CN=C2.C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.CC(N[C@H]1[C@@H](OP(OP(OC[C@H]2O[C@@H](N3C(=O)NC(=O)C=C3)[C@H](O)[C@@H]2O)(O)=O)(O)=O)O[C@H](CO)[C@@H](O)[C@@H]1O)=O.CCC(COC(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-].CCCC(NC1C2N=CN([C@@H]3O[C@@H]4COP(O)(O[C@H]4[C@H]3OC(CCC)=O)=O)C=2N=CN=1)=O.C1OP(O)(=O)O[C@H]2[C@@H](O)[C@H](N3C(Br)=NC4C(N)=NC=NC3=4)O[C@H]12.CCCCCCCCCCCCCC(O[C@H]1[C@@]2(OC(C)=O)C(C)(C)[C@H]2[C@@H]2C=C(CO)C[C@]3(O)C(=O)C(C)=C[C@H]3[C@@]2(O)[C@@H]1C)=O.OP([O-])(O)=O.[K+].C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.O=C[C@@H]([C@H]([C@H]([C@@H](CO)O)O)O)O>C(#N)C>[C:24]([NH:23][C@@H:14]1[C@@H:13]([OH:1])[C@H:19]([OH:20])[C@@H:18]([CH2:21][OH:22])[O:17][CH:15]1[OH:16])(=[O:25])[CH3:26]