Tromethamine hydrochloride → 2-Amino-2'-deoxyadenosine
Reaktionsgleichung
S(O)(O)(=O)=[O:2].[NH2:6][C:7]1[N:15]=[C:14]2[C:10]([NH:11][CH:12]=[N:13]2)=[C:9]([NH2:16])[N:8]=1.NC1N=[C:25]2[C:21](NC=N2)=C(N)N=1.C(O)[C:29](N)([CH2:32][OH:33])[CH2:30][OH:31].Cl>>[CH2:21]1[C@H:25]([N:13]2[C:14]3[N:15]=[C:7]([NH2:6])[N:8]=[C:9]([NH2:16])[C:10]=3[N:11]=[CH:12]2)[O:2][C@H:29]([CH2:30][OH:31])[C@H:32]1[OH:33]Komponenten
3
Versuchsvorschrift
The washed cells described above were added to 10 ml of 50 mM Tris-HCl buffer (pH 7.2) containing 100 mM 2′-deoxyribose-1-phosphoric acid and 100 mM 2,6-diaminopurine hemisulfate to a washed cell-concentration of 50 g/ and reacted at 60° C. for 2 hours. The concentration of 2,6-diaminopurine-2′-deoxyriboside produced in the reaction solution was measured by high performance liquid chromatography. The results are shown in Table 1.
Aufarbeitung
- customreacted at 60° C. for 2 hours