[C:1]([OH:6])(=[O:5])[CH:2]([CH3:4])[OH:3].[OH-].[Na+].[C:9]([O-:14])(=[O:13])[CH:10]([CH3:12])[OH:11].[S:15]([O-:19])([O-:18])(=[O:17])=[O:16].[Zn+2:20]>O>[C:1]([O-:6])(=[O:5])[CH:2]([CH3:4])[OH:3].[Zn:20].[C:9]([O-:14])(=[O:13])[CH:10]([CH3:12])[OH:11].[S:15]([O-:19])([O-:18])(=[O:17])=[O:16].[Zn+2:20]
This lactate-zinc sulate buffer solution is prepared by first dissolving 2.12g of an 85 percent solution of lactic acid in 500 ml of distilled water and adjusting the pH to 3.5 with 0.1N sodium hydroxide. This solution is then further diluted to give a total volume of one liter. A 100 ml aliquot of the lactate solution is mixed with 100 ml of a 0.1 M zinc sulfate solution and this mixture diluted to one liter to provide the final lactate-zinc sulfate buffer solution.
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