HCID 15932585, Verbindung → HCID 131717987, Verbindung

HRID 1588117Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1588117
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([O:32][C@@H:33]2[O:40][C@H:39]([CH2:41][OH:42])[C@@H:37]([OH:38])[C@H:35]([OH:36])[C@H:34]2[NH:43][C:44](=[O:46])[CH3:45])[C@@H:30]([CH2:47][OH:48])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:18]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([O:32][C@@H:33]4[O:40][C@H:39]([CH2:41][OH:42])[C@@H:37]([O:38][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:35]([OH:36])[C@H:34]4[NH:43][C:44](=[O:46])[CH3:45])[C@@H:30]([CH2:47][OH:48])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:29][C@H:30]([CH2:47][OH:48])[C@H:31]([OH:32])[C@H:16]([OH:15])[C@H:17]1[OH:49]

Komponenten

5

Edukt 2

HCID 15932585HCID 15932585, VerbindungHCID 54584734HCID 54584734, Verbindung

Produkt 1

HCID 131717987HCID 131717987, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 10313134Manganese(2+) dichlorideKatalysatorHCID 2724247Sodium CacodylateLösungsmittel

Versuchsvorschrift

A solution of compound 18 of Example 18 (60 mg), UDP-galactose, (145 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (5.25 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 47 mg. The structural identity of titled compound 24 was unanbiguously assigned by 1H- and 13C-n.m.r (see Tables 4 and 5, respectively).

Aufarbeitung

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

Quellen und Nachweise

ORD-Eintrag
ord-dc1755a4ffe84d0cad7055dad489b85c
Quelldatensatz
uspto-grants-1993_06
Patentnummer
US05220008

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