HCID 15932584, Verbindung → HCID 131717986, Verbindung

HRID 1656864Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1656864
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@@H:16]1[C@@H:31]([OH:32])[C@@H:30]([OH:33])[C@@H:29]([CH2:34][O:35][C@@H:36]2[O:43][C@H:42]([CH2:44][OH:45])[C@@H:40]([OH:41])[C@H:38]([OH:39])[C@H:37]2[NH:46][C:47](=[O:49])[CH3:48])[O:28][C@H:17]1[O:18][CH2:19][CH2:20][CH2:21][CH2:22][CH2:23][C:24]([O:26][CH3:27])=[O:25])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:17]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@@H:16]3[C@@H:31]([OH:32])[C@@H:30]([OH:33])[C@@H:29]([CH2:34][O:35][C@@H:36]4[O:43][C@H:42]([CH2:44][OH:45])[C@@H:40]([O:41][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:38]([OH:39])[C@H:37]4[NH:46][C:47](=[O:49])[CH3:48])[O:28][C@H:17]3[O:18][CH2:19][CH2:20][CH2:21][CH2:22][CH2:23][C:24]([O:26][CH3:27])=[O:25])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:28][C@H:29]([CH2:34][OH:35])[C@H:30]([OH:33])[C@H:31]([OH:32])[C@H:16]1[OH:15]

Komponenten

5

Edukt 2

HCID 15932584HCID 15932584, VerbindungHCID 54584734HCID 54584734, Verbindung

Produkt 1

HCID 131717986HCID 131717986, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 10313134Manganese(2+) dichlorideKatalysatorHCID 2724247Sodium CacodylateLösungsmittel

Versuchsvorschrift

A solution of compound 17 of Example 17 (72 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 95 mg. The structural identity of titled compound 23 was unambiguously assigned by 1H-- and 13C-n.m.r (see Tables 4 and 5, respectively).

Aufarbeitung

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

Quellen und Nachweise

ORD-Eintrag
ord-59d8c89d28334f48be6adf715ba4bcde
Quelldatensatz
uspto-grants-1993_10
Patentnummer
US05254676

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