Phosphate → Biotin

HRID 1768514

Reaktionsgleichung

Reaktionsgleichung von HRID 1768514
C1C(=O)N(OC(CCCCCN[C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@@H:23]2[S:27][CH2:26][C@@H:25]3[NH:28][C:29]([NH:31][C@H:24]23)=[O:30])=[O:18])=O)C(=O)C1.P([O-])([O-])([O-])=[O:33]>>[OH:33][C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@H:23]1[C@@H:24]2[C@@H:25]([NH:28][C:29]([NH:31]2)=[O:30])[CH2:26][S:27]1)=[O:18]

Komponenten

3

Edukt 2

HCID 1061PhosphateHCID 83874Nhs-LC-biotin

Produkt 1

HCID 171548Biotin

Versuchsvorschrift

OA (manufactured by Sigma Co., 30 mg) was dissolved in 0.1M phosphate buffer (pH 7.0 , 1 ml), thereto was added an NHS-LC-biotin solution (manufactured by Pierce Chemical Co., 12.6 mg/ml 0.1M phosphate buffer, pH 7.0; 100 μl) and the mixture was reacted at 30° C. for 1 hour. Thereafter, the procedure in Example 5 (a) was repeated to give a biotin-OA fraction.

Aufarbeitung

  1. customthe mixture was reacted at 30° C. for 1 hour

Quellen und Nachweise

ORD-Eintrag
ord-d5ecc4ec14794b97a139a22354e972dd
Quelldatensatz
uspto-grants-1995_01
Patentnummer
US05378608

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