Phosphate → beta-D-Galactopyranose

HRID 1768515

Reaktionsgleichung

Reaktionsgleichung von HRID 1768515
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].C1(=O)NC(=O)C=C1.P([O-])([O-])([O-])=O>>[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4]

Komponenten

3

Edukt 2

HCID 1061PhosphateHCID 131718819beta-Gal maleimide

Produkt 1

HCID 439353beta-D-Galactopyranose

Versuchsvorschrift

To the Fab' (1 mg) prepared in the above process (a) was added the β-Gal-maleimide (3.9 mg) prepared in the process (b) and the mixture was reacted at 30° C. for 30 minutes. The reaction solution was centrifuged and the supernatant was subjected to Sepharose 6B column (φ1.5 cm×50 cm, eluent: 10 mM phosphate buffer, pH 6.5, containing 0.1% BSA) to give a β-Gal-Fab' fragment.

Aufarbeitung

  1. customthe mixture was reacted at 30° C. for 30 minutes

Quellen und Nachweise

ORD-Eintrag
ord-46f60e02cd01433ea273c41e08592afb
Quelldatensatz
uspto-grants-1995_01
Patentnummer
US05378608

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