Alpha-Ketoglutarate → Oxalacetate

HRID 1769591

Reaktionsgleichung

Reaktionsgleichung von HRID 1769591
[NH2:1][C@H:2]([C:7]([O-:9])=[O:8])[CH2:3][C:4]([O-:6])=[O:5].[O:10]=[C:11]([CH2:15][CH2:16][C:17]([O-:19])=[O:18])[C:12]([O-:14])=[O:13]>>[C:4]([O-:6])(=[O:5])[CH2:3][C:2]([C:7]([O-:9])=[O:8])=[O:10].[NH2:1][C@H:11]([C:12]([O-:14])=[O:13])[CH2:15][CH2:16][C:17]([O-:19])=[O:18]

Komponenten

4

Edukt 2

HCID 164533Alpha-KetoglutarateHCID 5460541l-aspartic acid, sodium salt

Produkt 2

HCID 164550OxalacetateHCID 5460544L-glutamate(2-)

Versuchsvorschrift

PRINCIPLE ##STR5## AST catalyzes the transfer of the amino group from aspartate to 2-oxoglutarate to yield oxalacetate and glutamate. The oxalacetate is reduced to malate in the presence of malate dehydrogenase [MD] with the simultaneous oxidation of reduced nicotinamide adenine dinucleotide [NADH]. The rate of decrease in absorbance at 340 nm is directly proportional to AST activity.

Quellen und Nachweise

ORD-Eintrag
ord-01a7a9d20ed64ae891a8cf0d3bd44d71
Quelldatensatz
uspto-grants-1995_01
Patentnummer
US05382525

Verknüpfte Notizen

Wird geladen…