Tris(2-carboxyethyl)phosphine hydrochloride → Melittin

HRID 1888847Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1888847
N[C@H](C(O)=O)CS.[CH3:8][CH2:9][C@@H:10]([C@H:12]([NH:204][C:205]([CH2:207][NH2:208])=[O:206])[C:13]([NH:15][CH2:16][C:17]([NH:19][C@H:20]([C:22]([NH:24][C@H:25]([C:29]([NH:31][C@H:32]([C:37]([NH:39][C@H:40]([C:46]([NH:48][C@H:49]([C:53]([NH:55][C@H:56]([C:61]([NH:63][C@H:64]([C:68]([NH:70][C@H:71]([C:75]([NH:77][CH2:78][C:79]([NH:81][C@H:82]([C:87]([N:89]1[C@H:93]([C:94]([NH:96][C@H:97]([C:99]([NH:101][C@H:102]([C:107]([NH:109][C@H:110]([C:115]([NH:117][C@H:118]([C:121]([NH:123][C@H:124]([C:135]([NH:137][C@H:138]([C:143]([NH:145][C@H:146]([C:152]([NH:154][C@H:155]([C:163]([NH:165][C@H:166]([C:172]([NH:174][C@H:175]([C:183]([NH:185][C@H:186]([C:192]([NH:194][C@H:195]([C:201]([NH2:203])=[O:202])[CH2:196][CH2:197][C:198]([NH2:200])=[O:199])=[O:193])[CH2:187][CH2:188][C:189]([NH2:191])=[O:190])=[O:184])[CH2:176][CH2:177][CH2:178][NH:179][C:180]([NH2:182])=[NH:181])=[O:173])[CH2:167][CH2:168][CH2:169][CH2:170][NH2:171])=[O:164])[CH2:156][CH2:157][CH2:158][NH:159][C:160]([NH2:162])=[NH:161])=[O:153])[CH2:147][CH2:148][CH2:149][CH2:150][NH2:151])=[O:144])[C@H:139]([CH2:141][CH3:142])[CH3:140])=[O:136])[CH2:125][C:126]2[C:130]3[CH:131]=[CH:132][CH:133]=[CH:134][C:129]=3[NH:128][CH:127]=2)=[O:122])[CH2:119][OH:120])=[O:116])[C@H:111]([CH2:113][CH3:114])[CH3:112])=[O:108])[CH2:103][CH:104]([CH3:106])[CH3:105])=[O:100])[CH3:98])=[O:95])[CH2:92][CH2:91][CH2:90]1)=[O:88])[CH2:83][CH:84]([CH3:86])[CH3:85])=[O:80])=[O:76])[C@H:72]([OH:74])[CH3:73])=[O:69])[C@H:65]([OH:67])[CH3:66])=[O:62])[CH2:57][CH:58]([CH3:60])[CH3:59])=[O:54])[CH:50]([CH3:52])[CH3:51])=[O:47])[CH2:41][CH2:42][CH2:43][CH2:44][NH2:45])=[O:38])[CH2:33][CH:34]([CH3:36])[CH3:35])=[O:30])[CH:26]([CH3:28])[CH3:27])=[O:23])[CH3:21])=[O:18])=[O:14])[CH3:11].C(C(O)=O)CP(CCC(O)=O)CCC(O)=O.Cl>O>[CH3:8][CH2:9][C@@H:10]([C@H:12]([NH:204][C:205]([CH2:207][NH2:208])=[O:206])[C:13]([NH:15][CH2:16][C:17]([NH:19][C@H:20]([C:22]([NH:24][C@H:25]([C:29]([NH:31][C@H:32]([C:37]([NH:39][C@H:40]([C:46]([NH:48][C@H:49]([C:53]([NH:55][C@H:56]([C:61]([NH:63][C@H:64]([C:68]([NH:70][C@H:71]([C:75]([NH:77][CH2:78][C:79]([NH:81][C@H:82]([C:87]([N:89]1[C@H:93]([C:94]([NH:96][C@H:97]([C:99]([NH:101][C@H:102]([C:107]([NH:109][C@H:110]([C:115]([NH:117][C@H:118]([C:121]([NH:123][C@H:124]([C:135]([NH:137][C@H:138]([C:143]([NH:145][C@H:146]([C:152]([NH:154][C@H:155]([C:163]([NH:165][C@H:166]([C:172]([NH:174][C@H:175]([C:183]([NH:185][C@H:186]([C:192]([NH:194][C@H:195]([C:201]([NH2:203])=[O:202])[CH2:196][CH2:197][C:198]([NH2:200])=[O:199])=[O:193])[CH2:187][CH2:188][C:189]([NH2:191])=[O:190])=[O:184])[CH2:176][CH2:177][CH2:178][NH:179][C:180]([NH2:182])=[NH:181])=[O:173])[CH2:167][CH2:168][CH2:169][CH2:170][NH2:171])=[O:164])[CH2:156][CH2:157][CH2:158][NH:159][C:160]([NH2:162])=[NH:161])=[O:153])[CH2:147][CH2:148][CH2:149][CH2:150][NH2:151])=[O:144])[C@H:139]([CH2:141][CH3:142])[CH3:140])=[O:136])[CH2:125][C:126]2[C:130]3[CH:131]=[CH:132][CH:133]=[CH:134][C:129]=3[NH:128][CH:127]=2)=[O:122])[CH2:119][OH:120])=[O:116])[C@H:111]([CH2:113][CH3:114])[CH3:112])=[O:108])[CH2:103][CH:104]([CH3:105])[CH3:106])=[O:100])[CH3:98])=[O:95])[CH2:92][CH2:91][CH2:90]1)=[O:88])[CH2:83][CH:84]([CH3:85])[CH3:86])=[O:80])=[O:76])[C@H:72]([OH:74])[CH3:73])=[O:69])[C@H:65]([OH:67])[CH3:66])=[O:62])[CH2:57][CH:58]([CH3:60])[CH3:59])=[O:54])[CH:50]([CH3:52])[CH3:51])=[O:47])[CH2:41][CH2:42][CH2:43][CH2:44][NH2:45])=[O:38])[CH2:33][CH:34]([CH3:36])[CH3:35])=[O:30])[CH:26]([CH3:28])[CH3:27])=[O:23])[CH3:21])=[O:18])=[O:14])[CH3:11]

Komponenten

4

Edukt 2

HCID 2734570Tris(2-carboxyethyl)phosphine hydrochlorideHCID 131730796Cys Melittin

Produkt 1

HCID 16133648Melittin

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Versuchsvorschrift

Alternatively, in a 20 ml scintillation vial, Cys-Melittin (0.006 mmol, 1 ml) was reacted with 1.7 molar equivalents TCEP-HCl (0.010 mmol, 100 μl) and left to stir at room temperature for 30 min. MES-Na (400 μl) and water (390 μl) were added in amounts to yield final concentrations of 10 mg/ml Melittin and 20 mM MES-Na. The pH was checked and adjusted to pH 6.5-7. A solution of NAG-PEG8-Maleimide (100 mg/ml) was prepared in dH2O. NAG-PEG8-Maleimide (2 eq, 0.012 mmol, 110 μl) was added, and the solution was left to stir at room temperature for 48 h.

Aufarbeitung

  1. waitleft

Quellen und Nachweise

ORD-Eintrag
ord-6e66a2109a6a48eda4a88d8ecc1651ac
Quelldatensatz
uspto-grants-2013_08
Patentnummer
US08501930B2

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