Platinum → HCID 178443123, Verbindung

HRID 1954439

Reaktionsgleichung

Reaktionsgleichung von HRID 1954439
[Pt:1].[C:2]([O:19]C(=O)CCCCCCCCCCCCCCC)(=O)[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]>C(=O)(O)[O-].C([NH+](CC)CC)C.C(O)(C)C.O.[Pd]>[C:2]([Pt:1])(=[O:19])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]

Komponenten

6

Edukt 2

HCID 23939PlatinumHCID 69339Palmitic anhydride

Produkt 1

HCID 178443123HCID 178443123, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 3

HCID 23938PalladiumKatalysatorHCID 11768884Triethylammonium hydrogen carbonateLösungsmittelHCID 21889133Propan-2-ol--water (1/1)Lösungsmittel

Versuchsvorschrift

Preparation used the same procedure as the platinum cluster conjugate: an estimated 30 nmol of amino-derivatized 2-3 nm palladium cluster, dissolved in 0.6 M triethylammonium bicarbonate in 20% isopropanol/water, was evaporated to dryness five times from methanol, dissolved in 3 mL of dichloromethane, then treated with 200 nmol of palmitic anhydride. The mixture was stirred for one hour at room temperature, washed three times with 0.1 M sodium phosphate buffer at pH 6.5, evaporated to dryness, redissolved in 2:1 methanol/chloroform and separated by gel filtration (GH25, Amicon, eluted with 2:1 methanol:chloroform). After evaporation to dryness, lipid conjugation was demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate was shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent.

Aufarbeitung

  1. customPreparation
  2. customwas evaporated to dryness five times from methanol
  3. dissolutiondissolved in 3 mL of dichloromethane
  4. washwashed three times with 0.1 M sodium phosphate buffer at pH 6.5
  5. customevaporated to dryness
  6. dissolutionredissolved in 2:1 methanol/chloroform
  7. customseparated by gel filtration (GH25, Amicon
  8. washeluted with 2:1 methanol
  9. customAfter evaporation to dryness, lipid conjugation
  10. extractionwas demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate
  11. stirringwas shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent

Quellen und Nachweise

ORD-Eintrag
ord-cd88928292c946de8958728c9d98dd56
Quelldatensatz
uspto-grants-2000_09
Patentnummer
US06121425

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