[CH:1]1[N:6]=[C:5]([NH2:7])[C:4]2[N:8]=[CH:9][N:10]([C@@H:11]3[O:15][C@H:14]([CH2:16][O:17][P:18]([O:21][P:22]([O:25][P:26]([O-:29])([OH:28])=[O:27])([O-:24])=[O:23])([OH:20])=[O:19])[C@@H:13]([OH:30])[C@H:12]3[OH:31])[C:3]=2[N:2]=1.[Na+].[Na+].P([O-])([O-])([O-])=O.[K+].[K+].[K+]>>[P:18]([O:17][CH2:16][C@H:14]1[O:15][C@@H:11]([N:10]2[C:3]3[N:2]=[CH:1][N:6]=[C:5]([NH2:7])[C:4]=3[N:8]=[CH:9]2)[C@H:12]([OH:31])[C@@H:13]1[OH:30])([O:21][P:22]([O:25][P:26]([OH:28])([OH:29])=[O:27])([OH:24])=[O:23])(=[O:19])[OH:20]Adenosine-5'-triphosohate: ATP disodium salt (A 7699) was purchased from Sigma Chemical Company Ltd., Poole, Dorset U.K. Brain Heart Infusion Broth (BHI)--Unipath Ltd. was used to grow the inoculum. Serial dilutions of broth culture were made using 0.1M potassium phosphate buffer (pH 7).
Wird geladen…