Trifluoroacetic acid → Lys-Val-Lys

HRID 1967176

Reaktionsgleichung

Reaktionsgleichung von HRID 1967176
CC(OC([NH:8][CH2:9][CH2:10][CH2:11][CH2:12][C@H:13]([NH2:17])[C:14]([OH:16])=[O:15])=O)(C)C.[NH:18]([C:26]([O:28]CC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:19]([C:23]([OH:25])=O)[CH:20]([CH3:22])[CH3:21].[NH:43](C(OCC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:44](C(O)=O)[CH2:45][CH2:46][CH2:47][CH2:48][NH:49]C(OC(C)(C)C)=O.C(O)(C(F)(F)F)=O>CCOCC>[NH2:43][C@H:44]([C:26]([NH:18][C@H:19]([C:23]([NH:17][C@H:13]([C:14]([OH:16])=[O:15])[CH2:12][CH2:11][CH2:10][CH2:9][NH2:8])=[O:25])[CH:20]([CH3:21])[CH3:22])=[O:28])[CH2:45][CH2:46][CH2:47][CH2:48][NH2:49]

Komponenten

6

Edukt 4

HCID 6422Trifluoroacetic acidHCID 688217FMOC-L-valineHCID 2724628Fmoc-Lys(Boc)-OHHCID 2733283(2S)-2-amino-6-(((tert-butoxy)carbonyl)amino)hexanoic acid

Produkt 1

HCID 448140Lys-Val-Lys

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 3283Diethyl EtherLösungsmittel

Versuchsvorschrift

The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized.

Aufarbeitung

  1. washwashed
  2. custompurified by prep
  3. customHPLC after drying
  4. additionThe substance is diluted in 30 ml of dioxan
  5. additionwater=4:6, treated overnight with 2.0 g of BioRad resin (acetate form)
  6. filtrationfiltered

Quellen und Nachweise

ORD-Eintrag
ord-f65325c5211945f4a748c4af36745b30
Quelldatensatz
uspto-grants-2013_04
Patentnummer
US08420778B2

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