Sodium Chloride → HCID 178446018, Verbindung

HRID 1980277

Reaktionsgleichung

Reaktionsgleichung von HRID 1980277
C([NH:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:28])[CH3:27])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1)(=O)CNC(C1C=CC=CC=1)=O.[Cl-].[Na+].C[C@H](NC([C@H]1N(C([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N)CC(O)=O)=O)CCCNC(N)=N)=O)C(C)C)=O)CC2C=CC(O)=CC=2)=O)C(C)C)=O)CC2NC=NC=2)=O)CCC1)=O)C(O)=O>O>[NH2:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:27])[CH3:28])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1

Komponenten

5

Edukt 3

HCID 5234Sodium ChlorideHCID 178445996HCID 178445996, VerbindungHCID 178448346H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

Produkt 1

HCID 178446018HCID 178446018, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Versuchsvorschrift

50 μl of a solution containing 0.01 mole/liter of hippuryl-histidyl-leucine (substrate) and 0-100 μl of a test compound solution were added to 300 μl of a 0.2M tris-hydrochloric acid buffer solution containing 0.2 mole/liter of sodium chloride. The total volume of said mixture was adjusted to 450 μl with water. Then, 50 μl of angiotensin-converting enzyme (ACE) isolated from pig's renal cortex were added to the mixture, and the mixture was allowed to stand at 37° C. for 20 minutes. The amount of histidyl-leucine produced from the substrate by the action of ACE was assayed microbiologically by the use of Leuconostoc mesenteroides P-60, and the ACE-inhibitory activity of the test compound was estimated therefrom. The results are shown in the following Table 1.

Aufarbeitung

  1. customisolated from pig's renal cortex
  2. additionwere added to the mixture

Quellen und Nachweise

ORD-Eintrag
ord-e82be9ce65c8433cb41cf69a0fcbdf35
Quelldatensatz
uspto-grants-1985_04
Patentnummer
US04508727

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