Phosphate → D-Glucosamine
Reaktionsgleichung
P([O-])([O-])([O-])=O.C([NH:9][C@@H:10]1[C@@H:16]([OH:17])[C@H:15]([OH:18])[C@@H:14]([CH2:19][OH:20])[O:13][CH:11]1[OH:12])(=O)C>>[OH:12][CH:11]1[O:13][C@H:14]([CH2:19][OH:20])[C@@H:15]([OH:18])[C@H:16]([OH:17])[C@H:10]1[NH2:9]Komponenten
3
Versuchsvorschrift
An enzyme solution (0.1 ml) is added to 0.3 ml of 100 mM phosphate buffer (pH 7.8) containing 0.1 ml of 1% N-acetyl-D-glucosamine as a substrate, and the resultant mixture is incubated at 37° C. for 30 minutes. The amount of D-glucosamine formed is determined colorimetrically using indole-HCl according to the method of Z. Dische and E. Borenfreund [J. Biol. Chem., 184:517 (1950)]. One unit of the enzyme is defined as the amount of the enzyme which is capable of producing 1 μmol D-glucosamine from N-acetyl-D-glucosamine per minute at 37° C.
Quellen und Nachweise
- ORD-Eintrag
- ord-8963cbfe91a045aaa0bffa72e58f47f6
- Quelldatensatz
- uspto-grants-1995_06