Dicyclohexylcarbodiimide → Nitrilotriacetic Acid
Reaktionsgleichung
C1(N=C=NC2CCCCC2)CCCCC1.ON1C(=O)CCC1=O.[C:24]([CH2:27][N:28]([CH2:38][C:39]([OH:41])=[O:40])[C@H:29]([C:35]([OH:37])=[O:36])CCCCN)([OH:26])=[O:25]>>[N:28]([CH2:27][C:24]([OH:26])=[O:25])([CH2:29][C:35]([OH:37])=[O:36])[CH2:38][C:39]([OH:41])=[O:40]Komponenten
4
Versuchsvorschrift
10 mg of carboxy-modified carriers were washed twice with 1 ml of acetonitrile (MeCN) and then taken up in 1 ml of MeCN. To this were added 10 mmol of dicyclohexylcarbodiimide and 10 μmol of N-hydroxysuccinimide. This was followed by shaking at room temperature for two hours. Washing was then carried out once with 1 ml of cyclohexane and once with 1 ml of MeCN. The particles were then taken up in 1 ml of MeCN. 4 μmol of N,N-biscarboxymethyl-L-lysine were added thereto and shaken at room temperature for three hours. This was followed by washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0).
Aufarbeitung
- washwere washed twice with 1 ml of acetonitrile (MeCN)
- washWashing
- stirringshaken at room temperature for three hours
- washby washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0)
Quellen und Nachweise
- ORD-Eintrag
- ord-289785323577432daafbd57976916d0b
- Quelldatensatz
- uspto-grants-2008_05
- Patentnummer
- US07368295B2
Notizen zur Reaktion
Wird geladen…