(4S)-4-{[(benzyloxy)carbonyl]amino}-5-methoxy-5-oxopentanoic acid → Z-Glu-Ala-Ala-OH

HRID 2226821

Reaktionsgleichung

Reaktionsgleichung von HRID 2226821
[NH:1]([C:12]([O:14][CH2:15][C:16]1[CH:21]=[CH:20][CH:19]=[CH:18][CH:17]=1)=[O:13])[C@H:2]([C:8]([O:10]C)=O)[CH2:3][CH2:4][C:5](=[O:7])[OH:6].[NH2:22][C@H:23]([C:25]([NH:27][C@H:28]([C:30]([OH:32])=[O:31])[CH3:29])=[O:26])[CH3:24]>>[NH:1]([C:12]([O:14][CH2:15][C:16]1[CH:21]=[CH:20][CH:19]=[CH:18][CH:17]=1)=[O:13])[C@H:2]([C:8]([NH:22][C@H:23]([C:25]([NH:27][C@H:28]([C:30]([OH:32])=[O:31])[CH3:29])=[O:26])[CH3:24])=[O:10])[CH2:3][CH2:4][C:5](=[O:7])[OH:6]

Komponenten

3

Edukt 2

HCID 2733408(4S)-4-{[(benzyloxy)carbonyl]amino}-5-methoxy-5-oxopentanoic acid2×HCID 5484352L-Alanyl-L-alanine

Produkt 1

HCID 15730994Z-Glu-Ala-Ala-OH

Versuchsvorschrift

0.2 ml of aqueous solution containing 2 mM Z-Glu-OMe, 50 mMH-Ala-Ala-OH, 25 mMNaOH and 5 mg/ml endoproteinase Glu-C are deep-frozen. Subsequently the reaction mixture is kept at -25° C. until the Z-Glu-OMe is consumed. Following thawing, the yield is determined analytically by RP-HPLC and amounts to 76% of theory.

Aufarbeitung

  1. temperatureare deep-frozen
  2. customis consumed

Quellen und Nachweise

ORD-Eintrag
ord-fa470512c0aa482a82c110ca6bb61832
Quelldatensatz
uspto-grants-1994_09
Patentnummer
US05350682

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