Glycerol → Dodecenylsuccinic anhydride
Reaktionsgleichung
[CH2:1]([OH:8])[C:2](N)([CH2:5]O)[CH2:3]O.Cl.C[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH2:21]OS([O-])(=O)=O.[Na+].SC[CH2:30][OH:31].[OH:32]CC(CO)O>>[CH3:21][CH2:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][CH2:13][CH2:12]/[CH:11]=[CH:3]/[CH:2]1[C:1](=[O:8])[O:31][C:30](=[O:32])[CH2:5]1Komponenten
5
Versuchsvorschrift
A leaf of the transgenic plant was mixed with an extraction buffer solution (50 mM Tris-HCl, pH 6.8, 2% SDS, 6% 2-mercaptoethanol, 10% glycerol) having an amount eight times the weight of the leaf, followed by pulverization. The suspension was centrifuged at 12,000 rpm at 4° C. for 5 minutes, and thereafter, the supernatant was collected. The whole protein (10 μg) derived from each transformant was separated into individual proteins using 12.5% polyacrylamide gel, and the individual proteins were transferred onto Immobillon PVDF membrane (manufactured by Millopore).