Glycerol → Dodecenylsuccinic anhydride

HRID 2241176

Reaktionsgleichung

Reaktionsgleichung von HRID 2241176
[CH2:1]([OH:8])[C:2](N)([CH2:5]O)[CH2:3]O.Cl.C[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH2:21]OS([O-])(=O)=O.[Na+].SC[CH2:30][OH:31].[OH:32]CC(CO)O>>[CH3:21][CH2:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][CH2:13][CH2:12]/[CH:11]=[CH:3]/[CH:2]1[C:1](=[O:8])[O:31][C:30](=[O:32])[CH2:5]1

Komponenten

5

Edukt 4

HCID 753GlycerolHCID 15672-MercaptoethanolHCID 93573Tromethamine hydrochlorideHCID 3423265Sodium Lauryl Sulfate

Produkt 1

HCID 6433892Dodecenylsuccinic anhydride

Versuchsvorschrift

A leaf of the transgenic plant was mixed with an extraction buffer solution (50 mM Tris-HCl, pH 6.8, 2% SDS, 6% 2-mercaptoethanol, 10% glycerol) having an amount eight times the weight of the leaf, followed by pulverization. The suspension was centrifuged at 12,000 rpm at 4° C. for 5 minutes, and thereafter, the supernatant was collected. The whole protein (10 μg) derived from each transformant was separated into individual proteins using 12.5% polyacrylamide gel, and the individual proteins were transferred onto Immobillon PVDF membrane (manufactured by Millopore).

Aufarbeitung

  1. customthe supernatant was collected
  2. customwas separated into individual proteins

Quellen und Nachweise

ORD-Eintrag
ord-9f285c32f1c040d7a17edff7cc0cde14
Quelldatensatz
uspto-grants-2010_05
Patentnummer
US07723569B2

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