Indole-3-pyruvic acid → L-Tryptophan

HRID 2247795

Reaktionsgleichung

Reaktionsgleichung von HRID 2247795
[NH:1]1[C:9]2[C:4](=[CH:5][CH:6]=[CH:7][CH:8]=2)[C:3]([CH2:10][C:11](=O)[C:12]([OH:14])=[O:13])=[CH:2]1.CC1C(O)=C(CN)C(COP(O)(O)=O)=C[N:18]=1.P([O-])([O-])([O-])=O>O>[NH2:18][C@H:11]([C:12]([OH:14])=[O:13])[CH2:10][C:3]1[C:4]2[C:9](=[CH:8][CH:7]=[CH:6][CH:5]=2)[NH:1][CH:2]=1

Komponenten

5

Edukt 3

HCID 803Indole-3-pyruvic acidHCID 1053Pyridoxamine 5'-phosphateHCID 1061Phosphate

Produkt 1

HCID 6305L-Tryptophan

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Reaktionsbedingungen

Temperatur
37 °C

Versuchsvorschrift

To a solution containing 20.3 g (100 mmol) of indole-3-pyruvic acid and 26.5 g (100 mmol) of pyridoxamine-5-phosphate in 1000 ml of distilled water buffered at pH 6.5 with 0.5 M phosphate buffer and maintained at 37° C. is added 50 mg of the monoclonal antibodies prepared according to Example 15. The reaction mixture is gently agitated for 2 hours. The monoclonal antibodies are then recovered by ultrafiltration. Dialysis of the reaction mixture followed by lyophilization yields the product L-tryptophan.

Aufarbeitung

  1. additionis added 50 mg of the monoclonal antibodies
  2. customprepared
  3. filtrationThe monoclonal antibodies are then recovered by ultrafiltration
  4. customDialysis of the reaction mixture

Quellen und Nachweise

ORD-Eintrag
ord-75656fa1fc414ad1b532b6d1f7da6457
Quelldatensatz
uspto-grants-1991_08
Patentnummer
US05037750

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