Reaktionsgleichung
C(O)C(N)(CO)CO.Cl.C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.[CH3:30][CH2:31][CH2:32][CH2:33][CH2:34][C@H:35]([OH:54])/[CH:36]=[CH:37]/[C@@H:38]1[C@@H:42]([CH2:43]/[CH:44]=[CH:45]\[CH2:46][CH2:47][CH2:48][C:49]([OH:51])=[O:50])[C@H:41]2[O:52][O:53][C@@H:39]1[CH2:40]2.Cl.C(O)C(N)(CO)CO.CCCCC[C@H](O)/C=C/[C@H]1OC(O)C[C@H](O)[C@@H]1C/C=C\CCCC(O)=O>>[CH3:30][CH2:31][CH2:32][CH2:33][CH2:34][C@H:35]([OH:54])/[CH:36]=[CH:37]/[C@H:38]1[O:53][C@@H:39]2[O:52][C@@H:41]([CH2:40]2)[C@@H:42]1[CH2:43]/[CH:44]=[CH:45]\[CH2:46][CH2:47][CH2:48][C:49]([OH:51])=[O:50]Komponenten
7
Versuchsvorschrift
1 ml of a buffer (20 MM Tris-HCl buffer, 1 mM EDTA, pH 7.5) containing human blood platelet microsomes (50 μg protein/ml) and the test compound (final concentration 10−7M) was agitated, then incubated at 0° C. for 30 minutes. To this was added prostaglandin H2 (100 ng/2 μl). This was incubated at 23° C. for 3 minutes to cause a reaction. Next, 1M hydrochloric acid was added to make the solution acidic and stop the reaction, then this was neutralized by 1M Tris-Base and centrifuged at 3000 rpm for 20 minutes. The amount of the TxB2 in the supernatant was measured by the EIA method (Cayman Co. kit).
Aufarbeitung
- waitThis was incubated at 23° C. for 3 minutes
- customa reaction
- customthe reaction
- waitcentrifuged at 3000 rpm for 20 minutes
Quellen und Nachweise
- ORD-Eintrag
- ord-29c71b802d754eff95957135ab6dd020
- Quelldatensatz
- uspto-grants-2002_08
- Patentnummer
- US06433167B1
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