Reaktionsgleichung
P([O-])([O-])([O-])=O.[K+].[K+].[K+].C(O)[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.N[C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O.[CH:52]1[CH:57]=[N+:56]([C@@H:58]2[O:62][C@H:61]([CH2:63][O:64][P:65]([O:68][P:69]([O:72][CH2:73][C@H:74]3[O:78][C@@H:77]([N:79]4[C:83]5[N:84]=[CH:85][N:86]=[C:87]([NH2:88])[C:82]=5[N:81]=[CH:80]4)[C@H:76]([O:89][P:90]([OH:93])([OH:92])=[O:91])[C@@H:75]3[OH:94])([OH:71])=[O:70])([OH:67])=[O:66])[C@@H:60]([OH:95])[C@H:59]2[OH:96])[CH:55]=[C:54]([C:97]([NH2:99])=[O:98])[CH:53]=1.[Mg+2].[Cl-].[Cl-].C(OP(O)(O)=O)[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O.C(OP(O)(O)=O)[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O>>[CH:85]1[N:86]=[C:87]([NH2:88])[C:82]2[N:81]=[CH:80][N:79]([C@@H:77]3[O:78][C@H:74]([CH2:73][O:72][P:69]([O:68][P:65]([O:64][CH2:63][C@H:61]4[O:62][C@@H:58]([N:56]5[CH:55]=[C:54]([C:97]([NH2:99])=[O:98])[CH2:53][CH:52]=[CH:57]5)[C@H:59]([OH:96])[C@@H:60]4[OH:95])([OH:67])=[O:66])([OH:71])=[O:70])[C@@H:75]([OH:94])[C@H:76]3[O:89][P:90]([OH:93])([OH:92])=[O:91])[C:83]=2[N:84]=1Komponenten
Versuchsvorschrift
The activity of the enzyme is determined by the following procedure. That is, a total 100 ml buffer solution of 57 mM potassium phosphate containing 10.8 g of trehalose, 860 mg of glutathione and 17.2 mg of EDTA·2Na, pH 7.0, is prepared. A mixture of 1,400 μl of the buffer solution, 100 μl of 20 mM NADP+ aqueous solution, 100 μl of 26 mM MgCl2 aqueous solution, 100 μl of 1.34 mM glucose 1,6-diphosphate aqueous solution, 100 μl of 31 U/ml phosphoglucomutase aqueous solution, 100 μl of 35 U/ml glucose-6-phosphate dehydrogenase aqueous solution and 100 μl of a test sample enzyme solution is incubated at 30° C. and the amount of NADPH produced is determined by the absorbance at 340 nm with the passage of lime. The quantity of enzyme which produces one μ mole of NADPH in one minute under the above reaction conditions is made one Unit.
Aufarbeitung
- customis incubated at 30° C.
Quellen und Nachweise
- ORD-Eintrag
- ord-80191dca0a4f41b2ae534fbabbeefd78
- Quelldatensatz
- uspto-grants-1997_07
- Patentnummer
- US05643775
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