[CH3:1][CH:2]([NH2:5])[CH2:3][CH3:4].C(O)C(N)(CO)CO.Cl.[F:15][C:16]([F:28])([F:27])[C:17]1[CH:18]=C(CC(=O)C)[CH:20]=[CH:21][CH:22]=1.C1([C@H](N)C)C=CC=CC=1.C(O)(=O)CCCCCCC/C=C\CCCCCCCC>>[F:15][C:16]([F:28])([F:27])[C:17]1[CH:18]=[C:4]([CH2:3][C@H:2]([NH2:5])[CH3:1])[CH:20]=[CH:21][CH:22]=1The Arthrobacter species KNK168 strain was subjected to shaking culture in the same manner as in Example 6 using (RS)-1-methylpropylamine as an enzyme inducer. The cells harvested from one liter of the culture were suspended in one liter of a 0.1 M Tris-hydrochloric acid buffer (pH 8.5), and 30 g of 1-(3-trifluoromethylphenyl)-2-propanone, 18 g of (R)-1-phenylethylamine, and 44 g of oleic acid were added thereto. The components were reacted at 30° C. for 40 hours with stirring. After the reaction, the resulting mixture was subjected to extraction and distillation in the same manner as in Example 6, to give 19.5 g of (R)-1-(3-trifluoromethylphenyl)-2-aminopropane. The optical purity was 100% ee, and the yield was 65%.
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