Pyridine → Acridine

Temperatur

0 °C

To a cold, stirring methylene chloride solution (30 ml; 0° C.) of 2-chloro-4H-1,2,3-benzodioxaphosphorin-4-one (2.4 ml of 1M methylene chloride solution; 2.4 mmol) and pyridine (0.2 g; 2.4 mmol) was added the DMT-protected pseudonucleoside of Example 2, paragraph A (0.55 g; 0.8 mmol). After stirring at 0° C. for 1 hour, the reaction mixture was poured into 1M TEAB aqueous solution (60 ml). The organic solution was separated, dried, concentrated. The residue was purified by flash column chromatography, eluted with 1% Et3N/CH2Cl2, 1% Et3N/1%CH3OH/CH2Cl2. The combined fraction of the product was washed with 1M TEAB aqueous solution, dried over Na2SO4, and concentrated, affording 0.19 g, 65% of the phosphorylated DMT-protected acridine pseudonucleotide.

1. additionwas added the DMT-protected pseudonucleoside of Example 2, paragraph A (0.55 g; 0.8 mmol)
2. customThe organic solution was separated
3. customdried
4. concentrationconcentrated
5. customThe residue was purified by flash column chromatography
6. washeluted with 1% Et3N/CH2Cl2, 1% Et3N/1%CH3OH/CH2Cl2
7. washThe combined fraction of the product was washed with 1M TEAB aqueous solution
8. dry with materialdried over Na2SO4
9. concentrationconcentrated

ORD-Eintrag

ord-13abdcc7b4664668a3fd37e4965d21df

Quelldatensatz

uspto-grants-1995_05

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US05414077