Acetate → HCID 70619535, Verbindung

HRID 372778Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 372778
Cl.[NH2:2][C@H:3]([C:12]([NH:14][CH2:15][C:16]([NH:18][CH2:19][C:20]([NH:22][C@H:23]([C:34]([NH:36][C@H:37]([C:42]([OH:44])=[O:43])[CH2:38][CH2:39][S:40][CH3:41])=[O:35])[CH2:24][C:25]1[C:33]2[C:28](=[CH:29][CH:30]=[CH:31][CH:32]=2)[NH:27][CH:26]=1)=[O:21])=[O:17])=[O:13])[CH2:4][C:5]1[CH:10]=[CH:9][C:8]([OH:11])=[CH:7][CH:6]=1.C([O-])(=O)C>C(O)(=O)C>[C:42]([OH:44])(=[O:43])[CH3:37].[NH2:2][C@H:3]([C:12]([NH:14][CH2:15][C:16]([NH:18][CH2:19][C:20]([NH:22][C@H:23]([C:34]([NH:36][C@H:37]([C:42]([OH:44])=[O:43])[CH2:38][CH2:39][S:40][CH3:41])=[O:35])[CH2:24][C:25]1[C:33]2[C:28](=[CH:29][CH:30]=[CH:31][CH:32]=2)[NH:27][CH:26]=1)=[O:21])=[O:17])=[O:13])[CH2:4][C:5]1[CH:10]=[CH:9][C:8]([OH:11])=[CH:7][CH:6]=1

Komponenten

4

Edukt 2

HCID 175AcetateHCID 70620434HCID 70620434, Verbindung

Produkt 1

HCID 70619535HCID 70619535, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 176Acetic AcidLösungsmittel

Versuchsvorschrift

17.3 Parts L-tyrosylglycylglycyl-L-tryptophyl-L-methionine hydrochloride is dissolved in 250 parts by volume of 20% acetic acid and passed slowly through an IR-45 ion exchange column in the acetate form. The column is washed with 20% acetic acid until no more peptide is eluted. Fractions containing the product are combined and the solvent removed by stripping under reduced pressure at room temperature. The residual glass is dissolved in 75 parts water and lyophilized to give L-tyrosylglycylglycyl-L-tryptophyl-L-methionine acetic acid salt. This compound is represented by the following formula

Aufarbeitung

  1. washThe column is washed with 20% acetic acid until no more peptide
  2. washis eluted
  3. additionFractions containing the product
  4. customthe solvent removed
  5. customby stripping under reduced pressure at room temperature
  6. dissolutionThe residual glass is dissolved in 75 parts water

Quellen und Nachweise

ORD-Eintrag
ord-b6539617919848d1a54a7a3195315bce
Quelldatensatz
uspto-grants-1978_05
Patentnummer
US04092304

Notizen zur Reaktion

Wird geladen…