Diisopropylethylamine → N-[6-(1H-Indol-4-yl)-1H-indazol-4-yl]-5-methyl-2-(methyloxy)benzamide

To a solution of 5-methyl-2-(methyloxy)benzoic acid (67 mg, 0.40 mmol) in DMF (1 ml), HATU (248 mg, 0.40 mmol) and DIPEA (156 mg, 1.208 mmol) were added. The reaction was stirred for 30 mins under nitrogen before addition of 6-(1H-indol-4-yl)-1H-indazol-4-amine (50 mg, 0.20 mmol). The reaction was stirred for 3 h then it was loaded onto a PS-aminopropyl cartridge and left for 3 h. The cartridge was washed with 10% MeOH in DCM. Appropriate fractions were combined and the solvent was evaporated. The residue was dissolved in MeOH:DMSO (1 ml; 1:1, v/v) and purified by Mass Directed Automated Preparative HPLC using method B. The solvent was dried under a stream of nitrogen. The residue was dissolved in DMSO and further purified by HPLC using the following conditions:

1. waitleft for 3 h
2. washThe cartridge was washed with 10% MeOH in DCM
3. customthe solvent was evaporated
4. dissolutionThe residue was dissolved in MeOH
5. custom1:1, v/v) and purified by Mass Directed Automated Preparative HPLC
6. customThe solvent was dried under a stream of nitrogen
7. dissolutionThe residue was dissolved in DMSO
8. customfurther purified by HPLC

ORD-Eintrag

ord-418e8f9161d04a56b0b204662184b67a

Quelldatensatz

uspto-grants-2013_09

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US08536169B2