Ferulic Acid → Vanillin

HRID 430413

Reaktionsgleichung

Reaktionsgleichung von HRID 430413
C(O)(=O)/C=[CH:3]/[C:4]1[CH:12]=[CH:11][C:9]([OH:10])=[C:6]([O:7][CH3:8])[CH:5]=1.CC([O-])=[O:17].[Na+]>CO>[O:17]=[CH:3][C:4]1[CH:12]=[CH:11][C:9]([OH:10])=[C:6]([O:7][CH3:8])[CH:5]=1

Komponenten

4

Edukt 2

HCID 445858Ferulic AcidHCID 517045Sodium Acetate

Produkt 1

HCID 1183Vanillin

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 887MethanolLösungsmittel

Versuchsvorschrift

Metabolites of trans-ferulic acid, including the CoASH thioesters, were analysed and quantitated by HPLC using a Lichrosorb RP-18 column (20 cm×4.6 mm; Capital HPLC, Broxburn, West Lothian, UK) with a multiphasic gradient; solvent “A” was 20 mM NaOAc, adjusted to pH 6.0 and solvent “B” was MeOH; the flow rate was 1.2 ml/min; the proportion of solvent “B” rose linearly from 0% at 0 min to 10% at 15 min and thence to 50% at 40 min and 70% at 45 min, finally decreasing to 0% at 50 min. Detection was with a Spectra Focus detector (Thermo Separation Products, Stone, Staffs. UK), which permitted u/v spectral analysis of each eluting component. Typical approximate retention times were: CoASH, 3 min; vanillic acid, 7 min; trans-ferulic acid, 19 min; acetyl SCoA, 22 min; HMPHP SCoA, 29 min; vanilloyl SCoA, 31 min; vanillin, 31.5 min; trans-feruloyl SCoA, 34 min.

Aufarbeitung

  1. waitthence to 50% at 40 min
  2. wait70% at 45 min
  3. waitfinally decreasing to 0% at 50 min
  4. customDetection was with a Spectra Focus detector (Thermo Separation Products, Stone, Staffs. UK), which
  5. customCoASH, 3 min
  6. customvanillic acid, 7 min
  7. customtrans-ferulic acid, 19 min
  8. customacetyl SCoA, 22 min
  9. customHMPHP SCoA, 29 min
  10. customvanilloyl SCoA, 31 min
  11. customvanillin, 31.5 min
  12. customtrans-feruloyl SCoA, 34 min.

Quellen und Nachweise

ORD-Eintrag
ord-90298052baec4031997728998afbb97a
Quelldatensatz
uspto-grants-2001_11
Patentnummer
US06323011B1

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