Phosphate → Nadh

HRID 459736

Reaktionsgleichung

Reaktionsgleichung von HRID 459736
[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([OH:38])[C@@H:24]3[OH:39])([OH:20])=[O:19])([OH:16])=[O:15])[C@@H:9]([OH:40])[C@H:8]2[OH:41])[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH:2]=1.P([O-])([O-])([O-])=O.[I-].[Na+].[OH-].[Na+]>>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:41])[C@@H:9]4[OH:40])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:39])[C@H:25]3[OH:38])[C:32]=2[N:33]=1

Komponenten

5

Edukt 4

HCID 1061PhosphateHCID 5238Sodium IodideHCID 5893Nadide (JAN/USAN/INN)HCID 14798Sodium Hydroxide

Produkt 1

HCID 439153NadhAusbeute 19,5 %

Reaktionsbedingungen

Temperatur
23 °C

Versuchsvorschrift

NAD+ (34.0 mg, 38.0 μmol) was placed in 2 mL of a 0.1 M phosphate buffer containing 0.07 M sodium iodide. The pH of this solution was adjusted to 8.1 with NaOH and then placed in an 80 mL Fisher-Porter bottle. The Fisber-Porter bottle was evacuated and refilled with argon three times then [Cl2Ru(TPPTS)2]2 (7.7 mg, 2.9 μmol) was added under a flow of argon. The Fisher-Porter bottle was evacuated and filled with 70 psi of H2 and stirred to 23° C. for 13.0 hr. The volatiles were removed under reduced pressure and a sample dissolved in D2O. To this was added 20 μl of trimethylsilylpropionate standard solution. 1H NMR integration shows that 7.4 μmole of NADH have been produced under these conditions.

Aufarbeitung

  1. customThe Fisber-Porter bottle was evacuated
  2. additionrefilled with argon three times
  3. customThe Fisher-Porter bottle was evacuated
  4. additionfilled with 70 psi of H2
  5. customThe volatiles were removed under reduced pressure
  6. dissolutiona sample dissolved in D2O
  7. additionTo this was added 20 μl of trimethylsilylpropionate standard solution

Quellen und Nachweise

ORD-Eintrag
ord-7920297446904969b8e958793d185207
Quelldatensatz
uspto-grants-2007_03
Patentnummer
US07195889B2

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